Threadgill, DW, Fries, R, Faber, LK, Vassart, G, Gunawardana,
A, Stranzinger, G, and Womack, JE. 1990. The thyroglobulin gene
is syntenic with the MYC and MOS proto-oncogenes and carbonic anhydrase
II and maps to chromosome 14 in cattle. Cytogenetics Cell Genetics
53: 32-36.
Threadgill, DW, Wilkemeyer, M, Womack, JE, and Ledley, FD. 1990.
Localization of the murine methylmalonyl CoA mutase (Mut) locus
on chromosome 17 by in situ hybridization. Cytogenetics Cell Genetics
53: 112-114.
Threadgill, DW, and Womack, JE. 1990. Syntenic conservation between
humans and cattle I: human chromosome 9. Genomics 8:22-28.
Threadgill, DW, Adkison, LR, and Womack, JE. 1990. Syntenic conservation
between humans and cattle II: human chromosome 12. Genomics 8:29-34.
Threadgill, DW, and Womack, JE. 1990. Genomic analysis of the
major bovine milk protein genes. Nucleic Acids Research 18:6935-6942.
Threadgill, DW, and Womack, JE. 1991. Synteny mapping of human
chromosome 8 loci in cattle. Animal Genetics 22:117-122.
Gallagher, DS, Threadgill, DW, Jackson, AP, Parham, P, and Womack,
JE. 1991. Somatic cell mapping of bovine clathrin light chain genes:
identification of a new bovine syntenic group. Cytogenetics Cell
Genetics 56:154-156.
Fries, R, Threadgill, DW, Hediger, R, Gunawardana, A, Blessing,
M, Jorcano, JL, Stranzinger, G, and Womack, JE. 1991. Mapping of
bovine cytokeratin sequences to four different sites on three chromosomes.
Cytogenetics Cell Genetics 57:135-141.
Georges, M, Gunawardana, A, Threadgill, DW, Lathrop, M, Olsaker,
I, Mishra, A, Sargeant, L, Schoeberlein, A, Steele, M, Terry, C,
Threadgill, DS, Zhao, X, Holm, T, Fries, R, and Womack, JE. 1991.
Characterization of a set of variable number of tandem repeat markers
conserved in Bovidae. Genomics 11:24-32.
Bishop, MD, Tavakkol, A, Threadgill, DW, Simmen, FA, Simmen, RCM,
Davis, ME, and Womack, JE. 1991. Somatic cell mapping and restriction
fragment length polymorphism analysis of bovine insulin-like growth
factor 1 (IGF1). Journal of Animal Science 69:4306-4311.
Dietz, AB, Georges, M, Threadgill, DW, Womack, JE, and Schuler,
LA. 1992. Somatic cell mapping, polymorphism, and linkage analysis
of bovine prolactin-related proteins and placental lactogen. Genomics
14:137-143.
Nan, Z, Threadgill, DW, and Womack, JE. 1992. Synteny mapping
in the bovine: genes from human chromosome 4. Genomics 14:131-136.
Sharan, SK, Holdener-Kenny, B, Threadgill, DW, and Magunson, T.
1992. Genomic mapping within the albino-deletion complex using
individual early postimplantation mouse embryos. Mammalian Genome
3:79-83.
Gallagher, DS, Threadgill, DW, Ryan, AM, Womack, JE, and Irwin,
DM. 1993. Physical mapping of the lysozyme gene family in cattle.
Mammalian Genome 4:368-373.
Threadgill, DS, Threadgill, DW, Moll, YD, Weiss, JA, Zhang, N,
Davey, HW, Wildeman, AG, and Womack, JE. 1994. Syntenic assignment
of human chromosome 1 homologous loci in the bovine. Genomics 22:626-630.
Threadgill, DW, Dlugosz, AA, Hansen, L, Tennenbaum, T, Lichti,
U, Yee, D, LeMantia, C, Mourton, T, Herrup, K, Harris, RC, Barnard,
JA, Yuspa, SH, Coffey, RJ, and Magnuson, T. 1995. Targeted disruption
of mouse EGF-receptor: effect of genetic background on mutant phenotype.
Science 269:230-234.
Denning, MF, Dlugosz, AA, Threadgill, DW, Magnuson, T, and Yuspa,
SH. 1996. Activation of the epidermal growth factor receptor signal
transduction pathway stimulates tyrosine phosphorylation of protein
kinase Cd. Journal of Biological Chemistry 271:5325-5331.
Tong, BJ, Das, SK, Threadgill, DW, Magnuson, T, and Dey, SK. 1996.
Differential expression of the full-length and truncated forms
of the epidermal growth factor receptor in the preimplantation
mouse uterus and blastocyst. Endocrinology 137:1492-1496.
Hansen, LA, Alexander, N, Hogan, ME, Sundberg, JP, Dlugosz, A,Threadgill,
DW, Magnuson, T, and Yuspa, SH. 1997. Genetically null mice reveal
a central role for epidermal growth factor receptor in the maturation
of the hair follicle and normal hair development. American Journal
of Pathology 150:1959-1975
Threadgill, DW, Yee, D, Matin, A, Nadeau, JH, and Magnuson, T.
1997. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated
inbred strain. Mammalian Genome 8:390-393.
Threadgill, DW, Matin, A, Yee, D, Carrasquillo, MM, Henry, KR,
Rollins, KG, Nadeau, JH, and Magnuson, T. 1997. SSLPs to map genetic
differences between the 129 inbred strains and closed-colony random
bred CD-1 mice. Mammalian Genome 8:441-442.
Dlugosz, AA, Hansen, L, Cheng, C, Alexander, N, Denning, MF, Threadgill,
DW, Magnuson, T, Coffey, RJ, Yuspa, SH. 1997. Targeted disruption
of the epidermal growth factor receptor impairs growth of squamous
papillomas expressing the v-ras (Ha) oncogene but does not block
in vitro keratinocyte responses to oncogenic ras. Cancer Research
57:3180-3188.
Nishimura, H, Yerkes, E, Hohenfellner, K, Miyazaki, Y, Ma, J,
Hunley, TE, Yoshida, H, Ichiki, T, Threadgill, D, Phillips, JA
3rd, Hogan, BM, Fogo, A, Brock, JW 3rd, Inagami, T, Ichikawa, I.
1999. Role of the angiotensin type 2 receptor gene in congenital
anomalies of the kidney and urinary tract, CAKUT, of mice and men.
Molecular Cell 3:1-10.
Denning, MF, Dlugosz, AA, Cheng, C, Dempsey, PJ, Coffey, RJ, Threadgill,
DW, Magnuson, T, Yuspa, SH. 2000. Cross-talk between epidermal
growth factor receptor and protein kinase C during calcium-induced
differentiation of keratinocytes. Experimental Dermatology 9:192-199.
Reiter, JL, Threadgill, DW, Eley, GD, Strunk, KE, Danielsen, AJ,
Schell-Sinclair, C, Pearsall, RS, Green, PJ, Yee, D, Lampland,
AL, Balasubramaniam, S, Crossley, TO, Magnuson, TR, James, CD,
Maihle, NJ. 2001. Comparative genomic sequence analysis and isolation
of human and mouse alternative Egfr transcripts encoding truncated
receptor isoforms. Genomics 71:1-20.
Chaurand, P, DaGue, BB, Pearsall, RS, Threadgill, DW, Caprioli,
RM. 2001. Profiling proteins from azoxymethane-induced colon tumors
at the molecular level by MALDI mass spectormetry. Proteomics 1:1320-1326.
Roberts, RB, Min, L, Washington, MK, Olsen, SJ, Settle, S, Coffey,
RJ, Threadgill, DW. 2002. The epidermal growth factor receptor
is required for establishement of intestinal tumors in the ApcMin
mouse model. Proceedings of the National Academy of Science USA
99:1521-1526.
Threadgill, DW, Lu, L, Airey, D, Manly, K, Williams, RW. Extending
the utility of recombinant inbred strains through recombinant inbred
intercrosses (RIX). Submitted.
identification
and functional characterization of genes influencing an individual's
innate susceptibility to various diseases, in the interaction between
genes and the environment, and in the biological role of the Erbb
gene family. Although mouse genetics is our predominant research
tool, we are also actively using complementary molecular and proteomic
tools. The approach we are taking to study indiviudal variation is
through the integration of genetic, biologic, and engineering tools.
Genetics permits the detection and regional mapping of target genes.
Concurrently, we are gathering detailed molecular and proteomic data
about the biological variation. These studies will provide valuable
insights that will help in the identification of target genes. Lastly,
we are also experimenting with a new methods to genetically engineer
mice for the eventual validation of candidate genes. Representative
projects ongoing in our lab include:
